CDs are produced in a teamwork between students of the club and teachers of WUN group. Students of the club are responsible for CD production, teachers of WUN group are responsible for content of the CDs. All our CDs are focused on a hands-on lab experiment containing protocols for teachers and students. Each experiment is surrounded by teaching materials and background information.
If you are interested in a CD please write us an Email:
bt-ag@johanna-wittum-schule.de
CD XXIX: Nanopor Sequencing of the CRISPR/Cas9 cutted plasmid pBR322
With the self-produced CRISPR/Cas9 endonuclease, a targeted cut is inserted into the plasmid pBR322. The cut is detected both by gel electrophoresis and base-exact by Oxford Nanopore sequencing.
CD XXVIII: CRISPR/Cas9
CD 28 deals with a CRISPR/Cas9 in-vitro experiment to cut DNA of a phage at a selected nucleotide and the estimation of expected fragment lengths in gel electrophoresis.
CD XXVII: DNA-Isolation
CD 27 is focused on the experiment of DNA isolation out of human cheek cells.
CD XXVI: Epigenetic
CD 26 deals with the experiment of DNA-methylation and the proof of successful methylation by restriction fragment length polymorphism (RFLP).
CD: XXV: Production of lactic acid
CD 25 is focused on the production of lactic acid by Lactobacillus plantarum by fermentation in a professional- and self- made fermenter sustainable and degradable plastic PLA.
CD XXIV: Microbial Fuel cell
CD 24 deals with the energy production in a microbial fuel cell driven by yeast cells.
CD XXIII: Production of Biohydrogen
CD 23 deals with the production of biohydrogen out of molasses by fer-mentation and the proof of produced hydrogen in a fuel cell to run an engine.
CD XXII: Biogas Production
CD 22 is focused on the production of biogas out of fruit waste in an anaerobic fermentation followed by qualitative and quantitative estimation of gas compounds by gas chromatography.
CD XXI: Downstream Processing and Purity Test
CD 21 deals with the purification of GFP isolated out of E. coli by column chromatography and purity test of GFP by PAGE.
CD XX: Production of Bioethanol out of Paper Waste
CD 20 is focused on the experiment to produce bioethanol out of paper waste by anaerobic fermentation, it`s distillation and energy production in a fuel cell driven by bioethanol.
CD XIX: Downstream Processing
CD 19 is focused on the downstream processing of the enzyme DHFR out of a E. coli lysate and the following enzyme purity and activity test of DHFR.
CD XVIII: PCR
CD 18 (E-Book) deals with the detection of genetically modified food by PCR and gel electrophoresis.
CD XVII: Enzyme activity dependent on environmental factors
CD 17 is focused on experiments detecting enzyme activity of alcohol dehydrogenase dependent on pH, temperature etc.
CD XVI: Antibody Analytics
CD 16 deals with the immune precipitation and antibody analytics by polyacrylamide gel electrophoresis
CD XV: GFP Production
CD 15 deals with the production of the Green Fluorescing Protein (GFP) in E. Coli conducted in different professional fermenters.
CD XIV: Gen Regulation
CD 14 is focused on the gene regulation and production of GFP in E. coli.
CD XIII: Western-Blot
CD 13 is focused on the separation of muscle proteins actin and myosin by Western Blot.
CD XII: PCR
CD 12 deals with the detection of genetically modified plants by PCR and gel electrophoresis
CD XI: HIV Detection
CD 11 deals with the immunological detection of HIV by ELISA.
CD X: In-Vitro-Gene Expression
CD 10 is focused on in-vitro-gene expression of GFP and the estimation of molecular mass of produced protein.
CD IX: Column Chromatography
CD 9 describes the purification of a protein e. g. GFP out of a protein mixture by column chromatography.
CD VIII: Protein Fingerprinting
CD 8 describes the isolation of muscle proteins out of various fish species followed by a protein fingerprinting to detect evolutionary relationship between species.
CD VII: Genetical Modifications
CD 7 is focused on the science methods to detect genetical modified organisms and food by ELISA and PCR.
CD VI: ELISA
CD 6 deals with the detection of infected Petunia by the Petunia Flower Break Virus via ELISA.
CD V: Transformation of E. coli
CD 5 shows the transformation of E. coli with pGLO by heat shock treatment
CD IV: Immobilization of Enzymes
CD 4 deals with the immobilization of lactase and the estimation of enzyme activity of immobilized lactase.
CD III: PCR
CD 3 demonstrates the isolation of DNA out of human cheek cells and amplification of Alu repeats by PCR.
CD II: Plasmid Isolation and RFLP
CD 2 deals with the plasmid isolation out of bacteria and a following RFLP to produce a DNA fingerprint.
CD I: PCR
CD 1 deals with the amplification of Lambda Phage DNA conducted hands on in water baths.